2CLY
Subcomplex of the stator of bovine mitochondrial ATP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.499, 79.354, 115.665 |
| Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
| Resolution | 115.470 - 2.800 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.535 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.940 |
| Number of reflections | 21257 | |
| <I/σ(I)> | 19.7 | 2.4 |
| Completeness [%] | 94.1 | 77.4 |
| Redundancy | 11 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS, 150 MM NACL, 17% PEG 5K MME, 7.5% GLYCEROL, 1% PICOLINE, pH 8.00 |
