2CLO
Tryptophan Synthase (external aldimine state) in complex with (naphthalene-2'-sulfonyl)-2-amino-1-ethylphosphate (F19)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Collection date | 2005-02-14 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.730, 60.140, 67.510 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 19.930 - 1.500 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.450 | 1.550 |
| Rmerge | 0.140 | 0.630 |
| Number of reflections | 124324 | |
| <I/σ(I)> | 13.8 | 1.7 |
| Completeness [%] | 96.1 | 94.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | pH 7.8 |
