2CLF
Tryptophan Synthase in complex with N-(4'-trifluoromethoxybenzoyl)-2- amino-1-ethylphosphate (F6) - highF6 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.840, 58.989, 67.423 |
| Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
| Resolution | 19.150 - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.124 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.150 | 1.800 |
| High resolution limit [Å] | 1.600 | 1.700 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 90154 | |
| <I/σ(I)> | 11.9 | 3.2 |
| Completeness [%] | 95.4 | 73.3 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | pH 7.80 |
