2CL6

CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH S-caged GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-17
Detector	ADSC CCD
Spacegroup name	P 41
Unit cell lengths	69.097, 69.097, 35.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.010 - 1.240
R-factor	0.148
Rwork	0.146
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5p21
RMSD bond length	0.012
RMSD bond angle	1.625
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.010	1.260
High resolution limit [Å]	1.240	1.240
Rmerge	0.050	0.330
Number of reflections	46194
<I/σ(I)>	33.4	3.1
Completeness [%]	96.8	70.7
Redundancy	6.87	2.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291.15	PROTEIN SOLUTION: 64 MM TRIS PH 7.6, 20 MM MAGNESIUM CHLORIDE, 10 MM DTT, 0,1 MM SODIUM AZIDE; RESERVOIR SOLUTION: 100 MM HEPES PH 7.4, 200 MM MAGNESIUM ACETATE, 17% PEG 8000 (FRESHLY PREPARED) MIXTURE OF EQUAL VOLUMES OF PROTEIN AND RESERVOIR SOLUTION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.15K