2CL2
Endo-1,3(4)-beta-glucanase from Phanerochaete chrysosporium, solved using native sulfur SAD, exhibiting intact heptasaccharide glycosylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-05-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.302, 47.408, 152.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.270 - 1.350 |
| R-factor | 0.145 |
| Rwork | 0.143 |
| R-free | 0.16700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.714 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.100 |
| Number of reflections | 19367 | |
| <I/σ(I)> | 53.7 | 27.5 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 19 | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 20% PEG 3350, 0.2 M AMMONIUM NITRATE AT 20 DEG C, CONTROLLED HUMIDITY |
