2CKM

Torpedo californica acetylcholinesterase complexed with alkylene- linked bis-tacrine dimer (7 carbon linker)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-10-31
Detector	RIGAKU RAXIS4++
Spacegroup name	P 31 2 1
Unit cell lengths	111.884, 111.884, 137.413
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.270 - 2.150
R-factor	0.211
Rwork	0.211
R-free	0.24900
Structure solution method	OTHER
RMSD bond length	0.018
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.050	0.510
Number of reflections	80692
<I/σ(I)>	10.8
Completeness [%]	92.0	75.6
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8	277	28-33% V/V PEG 200, 0.5M MES PH5.8, 4DEG CELSIUS, SEEDING, pH 5.80