2CKK
High resolution crystal structure of the human kin17 C-terminal domain containing a kow motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2004-08-27 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.752, 46.309, 60.798 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.620 - 1.450 |
R-factor | 0.139 |
Rwork | 0.137 |
R-free | 0.17500 |
Structure solution method | SIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.519 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 1.810 |
Rmerge | 0.040 |
Number of reflections | 21818 |
<I/σ(I)> | 23.6 |
Completeness [%] | 95.0 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 290 | VAPOR DIFFUSION, SITTING DROP 10% ETHYLENE GLYCOL, 27% PEG 6000, 500 MM LICL, 100 MM SODIUM ACETATE PH 6.3 290 K |