2CJW
Crystal structure of the small GTPase Gem (GemDNDCaM) in complex to Mg.GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 116.617, 116.617, 81.409 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kao |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.271 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.342 |
| Number of reflections | 36826 | |
| <I/σ(I)> | 19 | 5.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M NACACODYLATE PH6.5, 0.9M NAACETATE, 5MM MGCL2 AND 2MM DTT |
