2CJR
Crystal structure of oligomerization domain of SARS coronavirus nucleocapsid protein.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.423, 84.203, 105.177 |
| Unit cell angles | 90.00, 131.18, 90.00 |
Refinement procedure
| Resolution | 29.800 - 2.500 |
| R-factor | 0.256 |
| Rwork | 0.256 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.300 |
| Number of reflections | 36262 | |
| <I/σ(I)> | 18.55 | 6.89 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.00 |
