2CIQ
Structure-based functional annotation: Yeast ymr099c codes for a D- hexose-6-phosphate mutarotase.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-20 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 106.190, 44.800, 73.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.160 - 1.700 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jov |
RMSD bond length | 0.012 |
RMSD bond angle | 1.397 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.410 |
Number of reflections | 39082 | |
<I/σ(I)> | 14.8 | 4.9 |
Completeness [%] | 98.4 | 98.2 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 25-30% POLYETHYLENE GLYCOL 3000, 0.2 M LITHIUM SULFATE, 0.1M HEPES PH 7.5 |