2CHP

Crystal structure of the dodecameric ferritin MrgA from B. subtilis 168

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 3
Unit cell lengths	125.370, 125.370, 125.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.710 - 2.000
R-factor	0.157
Rwork	0.155
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MRGA MUTANT
RMSD bond length	0.026
RMSD bond angle	1.772
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.710	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.320
Number of reflections	44572
<I/σ(I)>	8.6	2.2
Completeness [%]	95.4	88
Redundancy	4.4	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1