2CHI
Recombinant human H ferritin, K86Q and E27D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-02 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.528, 180.528, 180.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.080 - 1.600 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.030 | 0.250 |
| Number of reflections | 33262 | |
| <I/σ(I)> | 36 | 7.8 |
| Completeness [%] | 98.5 | 96.4 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP. RESERVOIR: MPD 5%, CACL2 4.7 MM, HEPES 50 MM PH 7.5 DROP: 1 UL PROTEIN (27MG/ML) AND 1 UL RESERVOIR |
