Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-15 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 59.292, 62.064, 74.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.470 - 1.000 |
| R-factor | 0.1 |
| Rwork | 0.100 |
| R-free | 0.11000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uqx |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.977 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.010 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 73738 | |
| <I/σ(I)> | 14.68 | 2.65 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 2.92 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 5UL DROPS OF 10MG/ML PROTEIN DISSOLVED IN WATER MIXED WITH 5UL DROPS OF 8% PEG 10000, 0.1M TRIS HCL, PH 8.2 WITH MANNOSE AT 270UG/ML |
