2CHC
Structure of Rv3472(D26N), a function unknown protein from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.339, 91.917, 191.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.320 - 1.690 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.755 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.790 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 60209 | |
| <I/σ(I)> | 24.67 | 5.23 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 6.5 | 5.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 293 | 0.1M HEPES PH7.4, 0.2M MGCL2, 25% PEG400, 20 DEGREES C, pH 7.40 |
