Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-02 |
| Detector | SIEMENS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.900, 67.580, 80.470 |
| Unit cell angles | 90.00, 105.69, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| Starting model (for MR) | 1CHB |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.730 |
| Data reduction software | MACRO |
| Data scaling software | MACRO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.246 |
| Number of reflections | 35318 | |
| Completeness [%] | 100.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 200 MM MGCL2 100 MM CACODYLATE 5% PEG 1000 PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | GM1 | 1 (mM) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | PEG1000 | 15 (%) |
