2CH7
Crystal structure of the cytoplasmic domain of a bacterial chemoreceptor from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.600, 99.400, 117.200 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.2587 |
| Rwork | 0.259 |
| R-free | 0.29710 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 29602 | |
| <I/σ(I)> | 21 | 3 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.2-2.6 M 1,6-HEXANEDIOL, 0.1 M NA ACETATE PH 4.5, 2-5 % ETHANOL OR ISOPROPANOL, 1 MM TRIMETHYL LEAD ACETATE |
