2CH6
Crystal structure of human N-acetylglucosamine kinase in complex with ADP and glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-01 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 135.710, 101.992, 110.181 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.800 - 2.720 |
R-factor | 0.231 |
Rwork | 0.227 |
R-free | 0.28900 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.414 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | HKL2Map |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.960 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.060 | 0.380 |
Number of reflections | 40858 | |
<I/σ(I)> | 20.2 | 3.8 |
Completeness [%] | 98.8 | 98.6 |
Redundancy | 6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 100 MM MES PH5.6, 100 MM NACL, 2% (W/V) PEG 20K, pH 5.60 |