2CH4
Complex between Bacterial Chemotaxis histidine kinase CheA domains P4 and P5 and receptor-adaptor protein CheW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 136.600, 136.600, 231.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1B3Q AND 1K0S |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.090 | 0.580 |
| Number of reflections | 27869 | |
| <I/σ(I)> | 40 | 10 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 4K 3-5%,NA ACETATE 0.1M PH 4.5,ADPNP 1MM |
