2CGQ
a putative acyl carrier protein(Rv0033) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.222, 45.222, 78.152 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.160 - 1.830 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t8k |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.160 | 1.950 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.030 | 0.200 |
| Number of reflections | 8555 | |
| <I/σ(I)> | 35.74 | 9.19 |
| Completeness [%] | 98.8 | 96.4 |
| Redundancy | 6.25 | 5.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PH6.5, 0.2M NACL, 25%(W/V)PEG3350, pH 6.50 |
