2CGL
Crystal Structure of L-rhamnulose kinase from Escherichia coli in complex with L-fructose, ADP and a modeled ATP gamma phosphate.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793, 0.9795, 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.781, 50.966, 158.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.340 - 1.880 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20900 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.529 |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 61818 | |
| <I/σ(I)> | 16.7 | 3.9 |
| Completeness [%] | 95.8 | 72.9 |
| Redundancy | 5.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17% PEG 8000, 120 MM LICL |
