2CGK
Crystal Structure of L-rhamnulose kinase from Escherichia coli in an open uncomplexed conformation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.259, 164.933, 51.570 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.460 |
| R-factor | 0.2382 |
| Rwork | 0.238 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cgl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 2.540 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.100 | 0.420 |
| Number of reflections | 29856 | |
| <I/σ(I)> | 8.3 | 3.6 |
| Completeness [%] | 95.1 | 97.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 17 % PEG 8000, 120 MM LICL |
