2CGF

A RADICICOL ANALOGUE BOUND TO THE ATP BINDING SITE OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-01-14
Detector	MARRESEARCH
Spacegroup name	P 43 2 2
Unit cell lengths	73.654, 73.654, 110.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.720 - 2.200
R-factor	0.17
Rwork	0.168
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LOCAL HSP90 N-TERM MODEL
RMSD bond length	0.017
RMSD bond angle	1.608
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.940	2.220
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.240
Number of reflections	15804
<I/σ(I)>	17.62	3.24
Completeness [%]	97.9	85.6
Redundancy	4.48	3.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1