2CG6
Second and third fibronectin type I module pair (crystal form I).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-24 |
| Detector | ADSC |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 37.855, 37.855, 108.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.740 - 1.550 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.23100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.696 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.740 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 12021 | |
| <I/σ(I)> | 6 | 1.8 |
| Completeness [%] | 98.2 | 93.2 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.2 M AMMONIUM SULPHATE, 0.1M NA ACETATE PH 4.5, 2% MPD, 5% PEG MME 550 |
