2CG3
Crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9796, 0.9790, 0.9256 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 86.100, 86.100, 364.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.540 - 2.600 |
| R-factor | 0.177 |
| Rwork | 0.173 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.520 |
| Number of reflections | 25870 | |
| <I/σ(I)> | 21.4 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 12% PEG4000,10% ISOPROPANOL, 0.2M LICL,0.1M CITRATE PH6, pH 6.00 |
