2CFY
Crystal structure of human thioredoxin reductase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 89.756, 149.651, 146.789 |
| Unit cell angles | 90.00, 91.96, 90.00 |
Refinement procedure
| Resolution | 149.070 - 2.700 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 100946 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 95.0 | 93 |
| Redundancy | 3.25 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 150 UL SITTING DROPS, 20% PEG3350, 0.1 M TRIS PH 7, ADDITIVE: NSDB-221 |
