2CFT

Crystal structure of human pyridoxal 5'-phosphate phosphatase with its substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-08
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	54.303, 54.303, 210.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.160 - 1.800
R-factor	0.187
Rwork	0.185
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.526
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800
Rmerge	0.040
Number of reflections	29341
<I/σ(I)>	50
Completeness [%]	96.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1