2CFP

Sugar Free Lactose Permease at acidic pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	123.920, 123.920, 190.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.980 - 3.300
R-factor	0.286
Rwork	0.286
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pv6
RMSD bond length	0.012
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.420
High resolution limit [Å]	2.950	3.300
Rmerge	0.180	0.590
Number of reflections	22915
<I/σ(I)>	4.4	1.3
Completeness [%]	83.2	75
Redundancy	2	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1