2CFF
Crystal Structure Of N-((5'-Phosphoribosyl)-Formimino)-5- Aminoimidazol-4-Carboxamid Ribonucleotid Isomerase mutant D127V (Ec 3. 1.3.15, Hisa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.710, 47.080, 105.250 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.200 |
| R-free | 0.28300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.080 |
| Number of reflections | 18703 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 90.3 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH8.5, 27% PEG 4000, 4.5% MPD, 0.2M MGCL2, pH 8.50 |
