2CFB

Glutamate-1-semialdehyde 2,1-Aminomutase from Thermosynechococcus elongatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-02-03
Detector	RIGAKU R-AXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	108.920, 108.920, 93.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.850
R-factor	0.176
Rwork	0.173
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsa
RMSD bond length	0.021
RMSD bond angle	1.826
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	EPMR
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.140	0.430
Number of reflections	24944
<I/σ(I)>	10.42	3.3
Completeness [%]	99.5	99.8
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		100 MM PIPES PH 6.5, 200 MM MAGNESIUM SULFATE, 17% PEG 3350