2CEU
Despentapeptide insulin in acetic acid (pH 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.152, 58.174, 54.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.130 - 1.800 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2-ZINC INSULIN |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.490 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.830 |
| High resolution limit [Å] | 1.830 | 1.800 |
| Rmerge | 0.040 | 0.070 |
| Number of reflections | 9014 | |
| <I/σ(I)> | 31 | 17 |
| Completeness [%] | 98.0 | 94 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 | 20 % ACETIC ACID, 0.05 M SODIUM SULPHATE, PH 2 |
