2CEQ
Beta-glycosidase from Sulfolobus solfataricus in complex with glucoimidazole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-17 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 167.927, 167.927, 93.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 145.860 - 2.140 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uwq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.353 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.100 | 0.400 |
Number of reflections | 83599 | |
<I/σ(I)> | 12.5 | 3.94 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 11-14 PEG 4K, 0.1 M SODIUM ACETATE, PH 4.6 0.2 AMMONIUM ACETATE, 10-13 MG/ML PROTEIN 25% ETHYLENE GLYCOL AS CRYO |