2CEI
Recombinant human H ferritin, K86Q mutant, soaked with Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-14 |
| Detector | MARRESEARCH |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 179.419, 179.419, 179.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.700 - 1.800 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.400 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.430 |
| Number of reflections | 21636 | |
| <I/σ(I)> | 15.6 | 3.3 |
| Completeness [%] | 92.0 | 95 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP. RESERVOIR: MPD 21%, CACL2 5.7 MM, HEPES 50 MM PH 7.5 DROP: 2 UL PROTEIN (4.5 MG/ML) AND 1 UL RESERVOIR |
