2CDY
Manganese Superoxide Dismutase (Mn-SOD) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.577, 87.100, 116.418 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.000 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2CE4 (MONOMER A) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.142 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.140 |
| Number of reflections | 48281 | |
| <I/σ(I)> | 31.8 | 9 |
| Completeness [%] | 82.2 | 41.7 |
| Redundancy | 9.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
