2CDP
Structure of a CBM6 in complex with neoagarohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS-4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.720, 54.996, 195.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.620 - 1.590 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cdo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.872 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.620 | |
| High resolution limit [Å] | 1.590 | |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 79215 | |
| <I/σ(I)> | 13.1 | 2.9 |
| Completeness [%] | 98.5 | 96.1 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2M NACL, 16-20% PEG 4000 BUFFERED TO PH 7.5 WITH TRIS/HCL |
