2CDM
The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 148.383, 148.383, 79.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.365 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 27451 | |
| <I/σ(I)> | 14.6 | 4.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 32 % PEG MME 2000, 0.3 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6 |
