2CCR

Structure of Beta-1,4-Galactanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	50.370, 80.640, 104.440
Unit cell angles	90.00, 99.29, 90.00

Refinement procedure

Resolution	19.970 - 2.300
R-factor	0.208
Rwork	0.208
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r8l
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.290
Number of reflections	34022
Completeness [%]	93.9	95.3
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				30-35%(W/V) PEG1500