2CCQ
The PUB domain functions as a p97 binding module in human peptide N-glycanase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 73.516, 73.516, 33.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.000 - 1.600 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.559 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.170 |
| Number of reflections | 13734 | |
| <I/σ(I)> | 18.4 | 6.7 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 5.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M SODIUM ACETATE, 0.1 M TRIS (PH 8.5), 30% PEG 4000 |
