2CCP

X-RAY STRUCTURES OF RECOMBINANT YEAST CYTOCHROME C PEROXIDASE AND THREE HEME-CLEFT MUTANTS PREPARED BY SITE-DIRECTED MUTAGENESIS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	104.930, 74.260, 45.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.200
R-factor	0.147
RMSD bond length	0.014
RMSD bond angle	0.037
Refinement software	JIMIN (WANG TO INCLUDE CONTRIBUTIO)

Data quality characteristics

	Overall
Rmerge	0.043 *
Number of reflections	16555 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	MPD	20 (%(v/v))
4	1	reservoir	MPD	30 (%(v/v))