2CC3
Structure of Agrobacterium tumefaciens VirB8 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.269, 72.349, 48.844 |
| Unit cell angles | 90.00, 110.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.239 |
| Rwork | 0.235 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.610 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | 0.290 |
| Number of reflections | 17972 | |
| <I/σ(I)> | 12.4 | 4.3 |
| Completeness [%] | 96.3 | 88.6 |
| Redundancy | 5.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M SODIUM ACETATE, PH 4.6, 0.2 M SODIUM BROMIDE, 20% MPD |
