2CBL

N-TERMINAL DOMAIN OF CBL IN COMPLEX WITH ITS BINDING SITE ON ZAP-70

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-05
Detector	MARRESEARCH
Spacegroup name	P 6
Unit cell lengths	123.380, 123.380, 56.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.173
Rwork	0.173
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CBL-N
RMSD bond length	0.013
RMSD bond angle	1.840
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.079	0.023
Total number of observations	35952 *
Number of reflections	20620
<I/σ(I)>	8.3	1.5
Completeness [%]	76.9	42.3
Redundancy	1.74	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1	22 *	pH 6.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%)
2	1	reservoir	calcium acetate	0.2 (M)
3	1	reservoir	dithiothreitol	2 (mM)
4	1	reservoir	sodium cacodylate	0.1 (M)