2CAY
Vps36 N-terminal PH domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.451, 88.375, 45.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.190 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.479 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.190 | 2.000 |
| High resolution limit [Å] | 1.600 | 1.900 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 31078 | |
| <I/σ(I)> | 5.34 | 1.55 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.54 | 3.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 1M AMSO4, 0.2M NACL, 6% GLYCEROL 0.1M TRIS PH 9.5 |
