2CAX
STRUCTURAL BASIS FOR COOPERATIVE BINDING OF RIBBON-HELIX-HELIX REPRESSOR OMEGA TO MUTATED DIRECT DNA HEPTAD REPEATS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.957, 44.655, 76.136 |
| Unit cell angles | 90.00, 109.26, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 14144 | |
| <I/σ(I)> | 12.1 | 2.5 |
| Completeness [%] | 88.6 | 68.8 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 150 MM NA/KPO4, PH 7.0, 2.4 M NA2MALONATE, PH 7.5, 2% AMINOCAPROIC ACID |
