2C9J
Structure of the fluorescent protein cmFP512 at 1.35A from Cerianthus membranaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.001, 60.108, 125.400 |
| Unit cell angles | 83.80, 89.98, 73.85 |
Refinement procedure
| Resolution | 55.000 - 1.350 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.558 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 300980 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 90.9 | 80 |
| Redundancy | 1.4 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG 4000, 0.1M TRIS PH 8.5, 0.2M MAGNESIUM CHLORIDE |
