2C9E
Peridinin-chlorophyll a protein, high-salt form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.576, 62.673, 63.345 |
| Unit cell angles | 90.00, 100.89, 90.00 |
Refinement procedure
| Resolution | 36.950 - 2.100 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PPR CHAIN M |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.370 |
| Number of reflections | 20528 | |
| <I/σ(I)> | 9.9 | 3.8 |
| Completeness [%] | 97.5 | 87.6 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8.5 % ISO-PROPANOL, 85 MM HEPES PH 7.5, 17 % PEG 4000, 15 % GLYCEROL |
