2C99
Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.250, 113.250, 39.332 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.900 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjw |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.951 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 22409 | |
| <I/σ(I)> | 22.7 | 2.9 |
| Completeness [%] | 97.6 | 92.4 |
| Redundancy | 6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 5% MPD |
