2C8R
insuline(60sec) and UV laser excited fluorescence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-24 |
| Detector | ADSC CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 78.188, 78.188, 78.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.930 - 1.500 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b2d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.124 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 12869 | |
| <I/σ(I)> | 30 | 10 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.1 | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | pH 9.00 |
