2C7Y
plant enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.154, 94.461, 111.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.210 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 44287 | |
| <I/σ(I)> | 12.8 | 2.2 |
| Completeness [%] | 96.7 | 80.6 |
| Redundancy | 3.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 25% POLYETHYLENE GLYCOL 4000, 0.1M TRIS-HCL PH 8.5, 300MM MGCL2. |
