2C7P
HhaI DNA methyltransferase complex with oligonucleotide containing 2- aminopurine opposite to the target base (GCGC:GMPC) and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-31 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 94.845, 94.845, 313.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.660 - 1.700 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mht |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.800 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.340 |
Number of reflections | 59771 | |
<I/σ(I)> | 7.5 | 2.2 |
Completeness [%] | 99.6 | 97.6 |
Redundancy | 11.8 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 50 MM NA CITRATE PH 5.6, 1.4 M AMMONIUM SULFATE |