2C7O
HhaI DNA methyltransferase complex with 13mer oligonucleotide containing 2-aminopurine adjacent to the target base (PCGC:GMGC) and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-18 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.180, 95.180, 312.547 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.080 - 1.900 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mht |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.200 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.200 |
Number of reflections | 43361 | |
<I/σ(I)> | 11.4 | 3.9 |
Completeness [%] | 99.6 | 98.5 |
Redundancy | 21.6 | 20.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 50 MM NA CITRATE PH 5.6, 1.6 M AMMONIUM SULFATE, 5 % GLUCOSE. |