2C7L
Low temperature structure of phycoerythrocyanin from Mastigocladus laminosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 63 |
| Unit cell lengths | 155.000, 155.000, 39.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.500 - 2.850 |
| R-factor | 0.2157 |
| Rwork | 0.216 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEE REMARK |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.610 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.530 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 0.240 |
| Number of reflections | 11512 | |
| <I/σ(I)> | 7.2 | 3.2 |
| Completeness [%] | 88.5 | 71.8 |
| Redundancy | 2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 5% PEG 4000, 5 MMOL/L POTASSIUM PHOSPHATE, PH 8.5, 4 DEG C, PEC 20 MG/ML, 5 MICO-LITER IN HANGING DROPS |
